mGluR5/1a Antibody [Unconjugated]

Western blot of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125 kDa monomers and the ~250 kDa dimers of mGluR1a and mGluR5.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

mGluR5/1a Antibody [Unconjugated] Summary

• Immunogen: Peptide from the C-terminus region of rat mGluR5 and mGluR1a
• Specificity: Rat ~125 kDa mGluR5 and mGluR1a monomers and ~250 kDa homodimers
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 dilution
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
• Buffer: 100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for mGluR5/1a Antibody [Unconjugated]

• mGluR5/1a

Background

Rat metabotropic glutamate receptor 5 (mGluR5b; also mGlu5b) is a 150 kDa, 1183 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. Although both mobilize intracellular Ca++ stores, mGluR1 also induces Ca++ influx via TRP channels. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks cell proliferation. mGluR5 has a large glycosylated extracellular domain (ECD) of 558 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There is one alternate splice form of mGluR5b. It shows a 32 aa deletion between aa 876 - 907 in the cytoplasmic region. The rat mGluR5 C-terminal cytoplasmic region is 90% and 97% aa identical to the equivalent regions in human and mouse mGluR5, respectively.

1. Pin, J-P. and F. Acher (2002) Curr. Drug Targets CNS Neurol. Disord. 1:297.
2. Boldyrev, A.A. et al. (2005) J. Neurochem. 95:913.
3. Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
4. Gama, L. et al. (2001) J. Biol. Chem. 276:39053.
5. Topolnik, L. et al. (2006) J. Physiol. 575:115.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.