Methylglyoxal Antibody (9E7) - BSA Free

Western Blot: Methylglyoxal Antibody (9E7) [NBP2-59368] - Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Immunocytochemistry/Immunofluorescence: Methylglyoxal Antibody (9E7) [NBP2-59368] - Tissue: Humnan embryonic kidney cells (HEK293). Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (SMC-516) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368] - Immunohistochemistry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Tissue: Heart. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:25 for 3 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Actin (C) Methylgyloxal Antibody (D) Composite.

Flow Cytometry: Methylglyoxal Antibody (9E7) [NBP2-59368] - Flow Cytometry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.

Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368] - Tissue: Kidney tissue. Species: Human. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Methylgyloxal Antibody (C) Composite.

• Reactivity: All-NA
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 9E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Methylglyoxal Antibody (9E7) - BSA Free Summary

• Immunogen: Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:50
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Methylglyoxal Antibody (9E7) - BSA Free

• 2-oxopropanal
• Pyruvaldehyde

Background

Methylglyoxal is the organic compound with the formula CH3C(O)CHO. Gaseous methylglyoxal has two carbonyl groups, an aldehyde and a ketone but in the presence of water, it exists as hydrates and oligomers. It is a reduced derivative of pyruvic acid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Methylglyoxal Antibody (NBP2-59368)(2)

Publications using NBP2-59368

• Nadia Pang, Ashang L. Laiva, Noof Z. Sulaiman, Priya Das, Fergal J. O’Brien, Michael B. Keogh, Avi Domb Dual Glyoxalase-1 and beta -Klotho Gene-Activated Scaffold Reduces Methylglyoxal and Reprograms Diabetic Adipose-Derived Stem Cells: Prospects in Improved Wound Healing Pharmaceutics 2024-02-13 [PMID: 38399319]
• Zhang WY, Zhao CM, Wang CS et al. Methylglyoxal accumulation contributes to accelerated brain aging in spontaneously hypertensive rats Free radical biology & medicine 2023-11-18 [PMID: 37984752] (WB)