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![Western Blot: Methylglyoxal Antibody (9E7) [NBP2-59368] - Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Methylglyoxal-Antibody-9E7-Western-Blot-NBP2-59368-img0007.jpg "Western Blot: Methylglyoxal Antibody (9E7) [NBP2-59368] - Western Blot analysis of Methylglyoxal-BSA Conjugate showing detection of 67 kDa Methylglyoxal protein using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Lane 1: Molecular Weight Ladder (MW). Lane 2: Methylglyoxal-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Methylglyoxal Antibody (9E7) [NBP2-59368] - Tissue: Humnan embryonic kidney cells (HEK293). Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (SMC-516) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Methylglyoxal-Antibody-9E7-Immunocytochemistry-Immunofluorescence-NBP2-59368-img0003.jpg)
![Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368] - Immunohistochemistry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Tissue: Heart. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:25 for 3 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Actin (C) Methylgyloxal Antibody (D) Composite.](http://images.novusbio.com/fullsize/Methylglyoxal-Antibody-9E7-Immunohistochemistry-NBP2-59368-img0008.jpg)
![Flow Cytometry: Methylglyoxal Antibody (9E7) [NBP2-59368] - Flow Cytometry analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7 (NBP2-59368). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody (NBP2-59368) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.](http://images.novusbio.com/fullsize/Methylglyoxal-Antibody-9E7-Flow-Cytometry-NBP2-59368-img0009.jpg)
![Immunohistochemistry: Methylglyoxal Antibody (9E7) [NBP2-59368] - Tissue: Kidney tissue. Species: Human. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Methylgyloxal Antibody (C) Composite.](http://images.novusbio.com/fullsize/Methylglyoxal-Antibody-9E7-Immunohistochemistry-NBP2-59368-img0006.jpg)