Methionine Sulfoxide Antibody

Product Discontinued

Methionine Sulfoxide Antibody Summary

• Immunogen: Oxidized methionine rich zein protein from Zea mays
• Specificity: Specific for all methionine sulfoxide containing proteins and peptides.
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is useful for Western blot. The size of the band is dependent on the size of the protein that has undergone methionine oxidation. It has also been used for immunohistochemistry on paraffin sections (rat brain with heat mediated antigen retrieval). Immunocytochemistry/Immunofluorescence was reported in scientific literature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Methionine Sulfoxide Antibody

• MetO

Background

Methionine (Met) oxidation is one of the most common posttranslational modifications to proteins that may alter protein structure and function. Accumulation of proteins containing methionine sulfoxide (MetO) is known to occur during oxidative stress. If the reversal of protein MetO to Met is not sufficient it may cause proteins to loose function, aggregate and be toxic to the cell. Methionine oxidation in proteins has been associated with neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and Creutzfeldt-Jakob disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Methionine Sulfoxide Antibody (NBP1-06707)(9)

Publications using NBP1-06707

• Riban V, Meunier J, Buttigieg D Et Al. In Vitro and In Vivo Neuroprotective Effects of Etifoxine in beta-amyloid-Induced Toxicity Models CNS Neurol Disord Drug Targets 2020-06-01 [PMID: 32479250]
• Minniti AN, Arriagada H, Zuniga S et al. Temporal pattern of neuronal insulin release during Caenorhabditis elegans aging: Role of redox homeostasis. Aging Cell. 2018-11-19 [PMID: 30456853] (WB, C. elegans)
• Gupta V, Mirzaei M, Gupta VB et al. Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin Sci Rep 2017-08-21 [PMID: 28827627] (WB, Human)
• Gostimskaya I, Grant CM. Yeast mitochondrial glutathione is an essential antioxidant with mitochondrial thioredoxin providing a back-up system. Free Radic. Biol. Med. 2016-02-17 [PMID: 26898146] (WB, Yeast)
• Doronina VA, Staniforth GL. Oxidative stress conditions increase the frequency of de novo formation of the yeast [PSI+] prion Mol Microbiol 2015-01-21 [PMID: 25601439]
• Nakaso K, Tajima N, Ito S et al. Dopamine-Mediated Oxidation of Methionine 127 in alpha-Synuclein Causes Cytotoxicity and Oligomerization of alpha-Synuclein. PLoS One 2013-01-01 [PMID: 23457458] (WB, Rat)
• Callizot N, Combes M, Steinschneider R, Poindron P. Operational dissection of beta-amyloid cytopathic effects on cultured neurons. J Neurosci Res 2013-05-01 [PMID: 23404368] (ICC/IF)
• Salama SA, Snapka RM. Amino Acid chloramine damage to proliferating cell nuclear antigen in Mammalian cells In Vivo 2012-07-01 [PMID: 22773562]
• Sideri TC, Koloteva-Levine N, Tuite MF, Grant CM. Methionine oxidation of Sup35 induces formation of the [PSI+] prion in a yeast peroxiredoxin mutant. J Biol Chem. 2011-08-09 [PMID: 21832086]

Reviews for Methionine Sulfoxide Antibody (NBP1-06707) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Rat.

reviewed by: Verified Customer IHC-P Rat 07/10/2009

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Rat brain
• Species: Rat
• Lot: A

reviewed by: Verified Customer WB Human 07/10/2009

Summary

• Application: Western Blot
• Sample Tested: Human brain human serum rat brain, Sample Amount: 15 ug
• Species: Human
• Lot: A

FAQs for Methionine Sulfoxide Antibody (NBP1-06707). (Showing 1 - 1 of 1 FAQs).

1. I would like to ask which protein was NBP1-06707 prepared against? Is there any species that the antibody is not compatible with?
   • The immunogen is from the zein protein of Zea mays which was oxidized and is methionine rich. Any known incompatible species will be listed on our datasheet but it shouldn't be species specific and should react with most species.