Mast Cell Protease-6/Mcpt6 Antibody (286820) [Unconjugated]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Clone
286820
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

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Mast Cell Protease-6/Mcpt6 Antibody (286820) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑6/Mcpt6
Ile32-Ser276
Accession # P21845
Specificity
Detects mouse Mast Cell Protease‑6/Mcpt6 in direct ELISAs and Western blots. In Western blots, 10% cross-reactivity with recombinant mouse (rm) MCP‑7 is observed and no cross-reactivity with rmMCP‑1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
Tpsb2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
MAB3736 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mast Cell Protease-6/Mcpt6 Antibody (286820) [Unconjugated]

  • Mast Cell Protease6
  • Mast Cell Protease-6
  • Mcpt6

Background

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast cell protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, Mcpt6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, Mcpt6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). Mcpt6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.

  1. Caughey, G. H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1535.
  2. Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
  3. Hallgren, J. and G. Pejler, (2006) FEBS J. 273:1871.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mast Cell Protease-6/Mcpt6 Antibody (MAB3736)(3)

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Bioinformatics

Gene Symbol Tpsb2
Uniprot