Mast Cell Protease-6/Mcpt6 Antibody (286820) [Unconjugated]

Treatment with a mast cell degranulation stabilizer completely inhibits the development of experimental COPD and increases in the levels of mast cell tryptase.Wild-type BALB/c mice (6–8 weeks old, female) were exposed to cigarette smoke (CS) for 8 weeks and treated with disodium cromoglycate (DSCG) intranasally from weeks 6–8. control mice received equal volumes of vehicle. DSCG completely inhibited the development of chronic airway inflammation, emphysema and impaired lung function in CS exposed mice. a Total leukocyte, b macrophage and c neutrophil numbers in bronchoalveolar lavage fluid (BALF) after 8 weeks of CS exposure (n = 8 per group). d Lung sections were stained with hematoxylin and eosin (scale bar = 50 μm), and e alveolar diameter was assessed as a measure of emphysema (n = 8 per group). f Lung function, in terms of transpulmonary resistance (n = 8 per group). g mMCP6 protein was assessed in whole mouse lung tissues by immunoblot (n = 8 per group), and h fold change of densitometry analysis of mMCP6 protein was normalized to beta -actin. Results are mean ± s.e.m, Statistical differences were determined using one-way ANOVA followed by Bonferroni post-test. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37963864), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IP
• Clone: 286820
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Mast Cell Protease-6/Mcpt6 Antibody (286820) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑6/Mcpt6
  Ile32-Ser276
  Accession # P21845
• Specificity: Detects mouse Mast Cell Protease‑6/Mcpt6 in direct ELISAs and Western blots. In Western blots, 10% cross-reactivity with recombinant mouse (rm) MCP‑7 is observed and no cross-reactivity with rmMCP‑1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Tpsb2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mast Cell Protease-6/Mcpt6 Antibody (286820) [Unconjugated]

• Mast Cell Protease6
• Mast Cell Protease-6
• Mcpt6

Background

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast cell protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, Mcpt6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, Mcpt6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). Mcpt6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.

1. Caughey, G. H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1535.
2. Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
3. Hallgren, J. and G. Pejler, (2006) FEBS J. 273:1871.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mast Cell Protease-6/Mcpt6 Antibody (MAB3736)(4)

Publications using MAB3736

• H Pettersson, B Zarnegar, A Westin, V Persson, C Peuckert, J Jonsson, J Hallgren, K Kullander SLC10A4 regulates IgE-mediated mast cell degranulation in vitro and mast cell-mediated reactions in vivo Sci Rep, 2017-04-24;7(1):1085. 2017-04-24 [PMID: 28439090] (ICC, Western Blot, Mouse)
• Kenny Roman, Joseph D. Done, Anthony J. Schaeffer, Stephen F. Murphy, Praveen Thumbikat Tryptase - PAR2 axis in Experimental Autoimmune Prostatitis, a model for Chronic Pelvic Pain Syndrome Pain 2014-07-01 [PMID: 24726923]
• Ugajin T, Kojima T, Mukai K, Obata K, Kawano Y, Minegishi Y, Eishi Y, Yokozeki H, Karasuyama H Basophils preferentially express mouse Mast Cell Protease 11 among the mast cell tryptase family in contrast to mast cells. J. Leukoc. Biol., 2009-12-01;86(6):1417-25. 2009-12-01 [PMID: 19703899] (Western Blot, Mouse)
• Tiwari N, Wang CC, Brochetta C, Ke G, Vita F, Qi Z, Rivera J, Soranzo MR, Zabucchi G, Hong W, Blank U VAMP-8 segregates mast cell-preformed mediator exocytosis from cytokine trafficking pathways. Blood, 2008-01-18;111(7):3665-74. 2008-01-18 [PMID: 18203950] (ICC, Mouse)

Bioinformatics

• Gene Symbol: Tpsb2
• Uniprot:
  • P21845()