Malondialdehyde Antibody (6H6) - BSA Free

Western Blot: Malondialdehyde Antibody (6H6) [NBP2-59366] - Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6 (NBP2-59366). Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 ug). Lane 3: Malondialdehyde-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug) . Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59366) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Immunocytochemistry/Immunofluorescence: Malondialdehyde Antibody (6H6) [NBP2-59366] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Flow Cytometry: Malondialdehyde Antibody (6H6) [NBP2-59366] - Flow Cytometry analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6 (NBP2-59366). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59366) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.

• Reactivity: All-NA
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 6H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Malondialdehyde Antibody (6H6) - BSA Free Summary

• Description: Novus Biologicals Mouse Malondialdehyde Antibody (6H6) - BSA Free (NBP2-59366) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Malondialdehyde Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Malondialdehyde Antibody (6H6) - BSA Free

• 3-Propanedial;Malonaldehyde;Malondialdehyde;MDA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Malondialdehyde Antibody (NBP2-59366)(2)

Publications using NBP2-59366

• Tang W, Chai W, Du D et al. The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury Oxidative medicine and cellular longevity 2022-02-16 [PMID: 35222805] (WB)
• Zhang J, Tucker LD, DongYan et al. Tert-butylhydroquinone post-treatment attenuates neonatal hypoxic-ischemic brain damage in rats Neurochem. Int. 2018-03-09 [PMID: 29530758] (ICC/IF)