MagCellect™ Plus Human CD44+ Cell Isolation Kit Summary
PRINCIPLE OF SELECTION
A mononuclear cell suspension is first incubated with the Human CD44 Biotinylated Antibody that targets the desired cells.
MagCellect(TM) Streptavidin (SA) Ferrofluid is next added to the reaction that allows the SA coated nanoparticles to interact with
the cells tagged with the monoclonal antibodies. The tube containing the cell suspension is then placed within a magnetic
field. Magnetically tagged cells will migrate toward the magnet (desired cell fraction), leaving the untagged cells (unwanted
cell population) in suspension to be harvested by aspiration while the tube remains in the magnetic field. The tube containing
the magnetically selected (desired) cells is then removed from the magnet and the cells are resuspended in MagCellect(TM) Plus
Buffer or tissue culture media. The enriched cell preparation is then available for a variety of applications including tissue
culture, immune status monitoring and flow cytometry.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
- Human CD44 AlexaFluor® 647Antibody 125ul
- Human CD44 Biotinylated Antibody 625ul
- Plus (10X) Buffer 100ml
- Streptavidin Ferrofluid 1.25ml
CD44 is a ubiquitously expressed receptor for hyaluronan and exerts control over cell growth, migration, and tumor progression. With variable N and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa. CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a platform for growth factors and metalloproteinases. It can function as a co-receptor that modifies activity of receptors including Met and the ErbB family tyrosine kinases. The intracellular domain of CD44 links the plasma membrane to the actin cytoskeleton via the ERM proteins, Ezrin, Radixin and Moesin. MagCellect products are designed for the isolation of cells in a liquid phase. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect( ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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