Macrophage galactose lectin Antibody (ER-MP23)

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications Flow, IHC, IHC-Fr
Clone
ER-MP23
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.1 mg/ml

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Macrophage galactose lectin Antibody (ER-MP23) Summary

Immunogen
The antibody reacts with the MGL antigen.
Specificity
The monoclonal antibody ER-MP23 specifically reacts with the macrophage galactose-specific lectin (MGL), a 38 kDa single chain surface glycoprotein. MGL is found on murine mature macrophages in the connective tissue neighboring epithelia of e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. MGL is present on the surface of connective tissue macrophages and their precursor cells in bone marrow. Also, MGL is expressed in macrophage cell lines (e.g. J774-1.6, RAW309Cr.1 and WR19M.1). Expression levels of MGL are increased in mature macrophages. The antigen is co expressed with the antigen recognized by monoclonal antibody BM8 (HM1066). The monoclonal antibody ER-MP23 has been raised after immunization of rats with mouse macrophage cell lines. Blocking studies demonstrated that the monoclonal antibody ER-MP23 is able to block mouse MGL.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MGL
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
The monoclonal antibody ER-MP23 can be used for immunohistology on frozen sections. Furthermore, the monoclonal antibody is useful for flow cytometry and for studies on inhibition of biological activity of the mouse macrophage galactose-specific lectin (MGL).For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For blocking studies in vitro, dilutions have to be made according to the amounts of MGL to be inactivated. The antigen is glutaraldehyde (0.05%) resistant.

Reactivity Notes

Mouse,Rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Background

Monoglyceride lipase is expressed in kidney, spleen, heart, liver, testis, stomach, brain and lung tissue, and adrenal gland. It is most abundantly expressed in skeletal muscle and adipose tissue. Endocannaboids such as arachidonoyl ethanolamide and 2-arachidonoyl glycerol function as short range modulators of cell and synaptic activity. Monoglyceride lipase hydrolyzes 2-arachidonoyl glycerol which terminates it's biological actions. Monoglyceride lipase also works consecutively with hormone sensitive lipase to mobilize fatty acids from triglyceride stores of adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MGL