Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138] - Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted [NBP2-48138] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

• Reactivity: Hu, Mu(-), Po(-), Rt(-)
• Applications: IHC
• Clone: D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Macrophage and Histiocytoma Marker Antibody (D11) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Membrane preparation from human hepatocytes
• Localization: Cell Surface & Cytoplasmic
• Marker: Macrophage & Histiocytoma Marker
• Specificity: In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this monoclonal antibody and a standard CD68 monoclonal antibody showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This monoclonal antibody shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This monoclonal antibody does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Molecular weight of antigen: 125kDa -135kDa Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Reactivity Notes

Does not react with Mouse, Rat or Porcine.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.