M13 Bacteriophage Protein g8p Antibody (RL-ph2) - BSA Free

Western Blot: M13 Bacteriophage Protein g8p Antibody (RL-ph2) [NBP3-09081] - RL-ph2 recognizing M13 phage coat protein g8p at 1:1000 dilution.

• Reactivity: V-Vi
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: RL-ph2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

M13 Bacteriophage Protein g8p Antibody (RL-ph2) - BSA Free Summary

• Description: The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
• Immunogen: RL-ph2 is a mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a Balb/c mouse immunized with isolated M13 phage coat proteins.
• Specificity: RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25 - 1:200
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot 1:100 - 1:1000
• Application Notes: This antibody is particularly useful for immunoblotting of separated phage proteins, and is also suitable for detection of M13 phages in multistep immunoblotting procedures, immunocytochemistry, flow cytometry, affinity chromatography and ELISA..
• Theoretical MW: 5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Only reacts with M13 phages and does not react with proteins of any animal species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Background

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P08648()