M13 Bacteriophage Protein g8p Antibody (RL-ph2)

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Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
RL-ph2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

M13 Bacteriophage Protein g8p Antibody (RL-ph2) Summary

Immunogen
Derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with isolated M13 phage coat proteins.
Specificity
RL-ph2 reacts with the major M13 filamentous phage coat protein g8p.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:1000
  • ELISA
  • Flow Cytometry 1:25-1:200
  • Immunocytochemistry/Immunofluorescence
Theoretical MW
5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Only reacts with M13 phages and does not react with proteins of any animal species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Notes

Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Background

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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