M13 bacteriophage Antibody (E1) [Alexa Fluor™ Plus 594]

• Reactivity: V-Vi
• Applications: WB, Flow, IHC
• Clone: E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

M13 bacteriophage Antibody (E1) [Alexa Fluor™ Plus 594] Summary

• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.
• Specificity: This antibody is specific for the M13 bacteriophage protein coat, amongst others the g3p protein.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

M13 phage

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for M13 bacteriophage Antibody (E1) [Alexa Fluor™ Plus 594]

• Coat protein A; Coat protein B; Coat protein C polypeptide I; Coat protein C polypeptide II; Coat protein D; G3P; III; IX; M13 coat protein A; M13 coat protein B; M13 coat protein C polypeptide I; M13 coat protein C polypeptide II; M13 coat protein D; M13

Background

Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage.May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.