Ly49C Antibody (14B11) - Azide and BSA Free

Flow Cytometry: Ly49C Antibody (14B11) [NBP1-28082] - Analysis of splenocytes by multiple staining.

• Reactivity: Mu
• Applications: Flow
• Clone: 14B11
• Clonality: Monoclonal
• Host: Golden Syrian Hamster
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Ly49C Antibody (14B11) - Azide and BSA Free Summary

• Description: Novus Biologicals Golden Syrian Hamster Ly49C Antibody (14B11) - Azide and BSA Free (NBP1-28082) is a monoclonal antibody validated for use in Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: IL-2 activated natural killed (A-LAK) cells from C57BL/6 mice
• Specificity: An epitope common to the Ly49C, Ly49F, Ly49H and Ly49I allelic/splice variants
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Golden Syrian Hamster
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Ly49C Antibody (14B11) - Azide and BSA Free

• 5E6
• Cmv1
• Killer cell lectin like receptor 3
• Killer cell lectin like receptor 6
• Killer cell lectin like receptor 8
• Killer cell lectin like receptor 9
• Klra3
• Klra6
• Klra8
• Klra9
• Ly49 C antigen
• Ly49 F antigen
• Ly49 H antigen
• Ly49 I antigen
• Lymphocyte antigen 49C
• Lymphocyte antigen 49F
• Lymphocyte antigen 49H
• Lymphocyte antigen 49I
• T cell surface glycoprotein Ly 49C
• T cell surface glycoprotein Ly 49F
• T cell surface glycoprotein Ly 49H
• T cell surface glycoprotein Ly 49I

Background

The monoclonal antibody 14B11 reacts with murine Ly49C, Ly49F, Ly49H and Ly49I, members of the lectin-like homodimeric Ly49 family of cell surface receptors. (1) Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. (2) These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells. The 14B11 antibody stains approx. 90% of NK1.1+CD3- splenocytes, and approx. 6% of CD8+ spleen cells from C57BL/6 mice. (1) The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.