LTB4 Parameter Assay Kit

• Reactivity: All-Multi
• Applications: ELISA
• Conjugate: Biotin

LTB4 Parameter Assay Kit Summary

• Background: The Parameter LTB₄ Immunoassay is a 4.5 hour forward sequential competitive enzyme immunoassay designed to measure LTB₄ in cell culture supernates, serum, and plasma.
• Specificity: Measures LTB₄ levels in various samples
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LTB4 Parameter Assay Kit

• Leukotriene B4
• LTB4

Background

Leukotriene B4 (LTB4) is a major product of arachidonic acid metabolism and is formed via the 5-lipoxygenase pathway. LTB4 stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Leukotriene B4/LTB4 (KGE006B)(52)

Publications using KGE006B

• Kwon, WY;Jung, YS;Suh, GJ;Kim, SH;Lee, A;Kim, JY;Kim, H;Park, H;Shin, J;Kim, T;Kim, KS;Itagaki, K;Hauser, CJ; Removal of circulating mitochondrial N-formyl peptides via immobilized antibody therapy restores sepsis-induced neutrophil dysfunction Journal of leukocyte biology 2024-08-06 [PMID: 39107254] (Human)
• Kim, H;Jung, J;Lee, M;Kim, M;Kang, N;Kim, OK;Lee, J; Curcuma longa L. extract exhibits anti-inflammatory and cytoprotective functions in the articular cartilage of monoiodoacetate-injected rats Food & nutrition research 2024-03-19 [PMID: 38571919] (Rat)
• Choi, YJ;Jung, JI;Bae, J;Lee, JK;Kim, EJ; Evaluating the Anti-Osteoarthritis Potential of Standardized Boswellia serrata Gum Resin Extract in Alleviating Knee Joint Pathology and Inflammation in Osteoarthritis-Induced Models International journal of molecular sciences 2024-03-12 [PMID: 38542192] (Rat)
• Kim, J;Ryu, G;Seo, J;Go, M;Kim, G;Yi, S;Kim, S;Lee, H;Lee, JY;Kim, HS;Park, MC;Shin, DH;Shim, H;Kim, W;Lee, SY; 5-aminosalicylic acid suppresses osteoarthritis through the OSCAR-PPAR? axis Nature communications 2024-02-03 [PMID: 38310093] (Mouse)
• Li, W;Xia, Y;Yang, J;Sanyal, AJ;Shah, VH;Chalasani, NP;Yu, Q; Disrupted balance between pro-inflammatory lipid mediators and anti-inflammatory specialized pro-resolving mediators is linked to hyperinflammation in patients with alcoholic hepatitis medRxiv : the preprint server for health sciences 2023-12-17 [PMID: 38168393] (Human)
• Adams, JM;Rege, SV;Liu, AT;Vu, NV;Raina, S;Kirsher, DY;Nguyen, AL;Harish, R;Szoke, B;Leone, DP;Czirr, E;Braithwaite, S;Kerrisk Campbell, M; Leukotriene A4 hydrolase inhibition improves age-related cognitive decline via modulation of synaptic function Science advances 2023-11-15 [PMID: 37976357] (Mouse)
• Hein, LE;SenGupta, S;Gunasekaran, G;Johnson, CN;Parent, CA; TGF-?1 activates neutrophil signaling and gene expression but not migration PloS one 2023-09-08 [PMID: 37682817] (Human)
• Hein, LE;SenGupta, S;Gunasekaran, G;Johnson, C;Parent, CA; TGF-?1 activates neutrophil signaling and gene expression but not migration bioRxiv : the preprint server for biology 2023-05-27 [PMID: 37292899] (Human)
• C Sun, W Zheng, L Liang, Z Zhang, Z Liu, W Sun, J Zhou, W Qiao, Q Xie, R Tang Acute Coronary Syndrome May be Associated with Decreased Resolvin D1-to-Leukotriene B4 Ratio International heart journal, 2023-01-23;0(0):. 2023-01-23 [PMID: 36682769] (Human)
• J Rodríguez-, JM Torres-Are, N Ávila-Vanz, R Springall, R Bojalil, O Infante, C Lerma, JC Echeverría Association of the Heart Rate Variability Response to Active Standing with the Severity of Calcific Aortic Valve Disease: Novel Insights of a Neurocardiovascular Pathology Journal of Clinical Medicine, 2022-08-16;11(16):. 2022-08-16 [PMID: 36013009] (Human)
• R Khazen, B Corre, Z Garcia, F Lemaître, S Bachellier, C d'Enfert, P Bousso Spatiotemporal dynamics of calcium signals during neutrophil cluster formation Oncogene, 2022-07-12;119(29):e2203855119. 2022-07-12 [PMID: 35858359] (Mouse)
• KH Lee, NF Ali, SH Lee, Z Zhang, M Burdick, ZJ Beaulac, G Petruncio, L Li, J Xiang, EM Chung, KW Foreman, SM Noble, YM Shim, M Paige Substrate-dependent modulation of the leukotriene A4 hydrolase aminopeptidase activity and effect in a murine model of acute lung inflammation Oncogene, 2022-06-08;12(1):9443. 2022-06-08 [PMID: 35676292] (Mouse)
• M Zheng, P Fan, P Yang, J Zheng, D Zhao Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p Journal of Immunology Research, 2022-05-20;2022(0):4086710. 2022-05-20 [PMID: 35637792] (Human)
• SH Jiang, D Liu, LP Hu, S Zhang, Y Yu, YW Sun, J Ji, ZG Zhang Modeling of cancer-related body-wide effects identifies LTB4 as a diagnostic biomarker for pancreatic cancer EBioMedicine, 2022-05-10;80(0):104050. 2022-05-10 [PMID: 35561453] (Human)
• HB Lee, MJ Oh, M Ho Do, Y Kim, I Choi, YS Kim, HY Park Dietary rhamnogalacturonan-? rich extracts of molokhia ameliorate high fat diet-induced obesity and gut dysbiosis The Journal of nutritional biochemistry, 2022-02-01;0(0):108954. 2022-02-01 [PMID: 35121021] (Mouse)
• JJ Shi, HF Liu, T Hu, X Gao, YB Zhang, WR Li, Q Wang, SJ Zhang, D Tang, YB Chen Danggui-Shaoyao-San improves cognitive impairment through inhibiting O-GlcNAc-modification of estrogen alpha receptor in female db/db mice Journal of ethnopharmacology, 2021-08-23;281(0):114562. 2021-08-23 [PMID: 34438027] (Mouse)
• Duo-Yao Cao, Jorge F. Giani, Luciana C. Veiras, Ellen A. Bernstein, Derick Okwan-Duodu, Faizan Ahmed et al. An ACE inhibitor reduces bactericidal activity of human neutrophils in vitro and impairs mouse neutrophil activity in vivo Science Translational Medicine 2021-07-28 [PMID: 34321319] (Mouse)
• X Luo, X Huo, Y Zhang, Z Cheng, S Chen, X Xu Increased intestinal permeability with elevated peripheral blood endotoxin and inflammatory indices for e-waste lead exposure in children Chemosphere, 2021-05-15;279(0):130862. 2021-05-15 [PMID: 34134434] (Human)
• KA Babatunde, X Wang, A Hopke, N Lannes, PY Mantel, D Irimia Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells Scientific Reports, 2021-01-12;11(1):778. 2021-01-12 [PMID: 33436661] (Human)
• R Lin, Y Zhang, K Pradhan, L Li TICAM2-related pathway mediates neutrophil exhaustion Sci Rep, 2020-09-01;10(1):14397. 2020-09-01 [PMID: 32873853] (Mouse)
• C Olesch, E Sirait-Fis, M Berkefeld, AF Fink, RM Susen, B Ritter, BE Michels, D Steinhilbe, FR Greten, R Savai, K Takeda, B Brüne, A Weigert S1PR4 ablation reduces tumor growth and improves chemotherapy via CD8+ T cell expansion J. Clin. Invest., 2020-10-01;0(0):. 2020-10-01 [PMID: 32663191] (Mouse)
• AD Allen, AM Bassil, DJ Berkoff, M Al Maliki, RW Draeger, PS Weinhold Minocycline microspheres did not significantly improve outcomes after collagenase injection of tendon J Orthop, 2019-06-03;16(6):580-584. 2019-06-03 [PMID: 31660026] (Rat)
• S Geng, Y Zhang, C Lee, L Li Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis Sci Adv, 2019-02-06;5(2):eaav2309. 2019-02-06 [PMID: 30775441] (Mouse)
• E? Alexopoulo, G Haritos, C Befani, G Malakasiot, VA Lachanas, P Liakos, K Gourgoulia, AG Kaditis Serum leukotriene B4 levels, tonsillar hypertrophy and sleep-disordered breathing in childhood Int. J. Pediatr. Otorhinolaryngol., 2018-08-07;113(0):218-222. 2018-08-07 [PMID: 30173989] (Human)
• M Plagge, T Laskay Early Production of the Neutrophil-Derived Lipid Mediators LTB4 and LXA4 Is Modulated by Intracellular Infection with Leishmania major Biomed Res Int, 2017-10-18;2017(0):2014583. 2017-10-18 [PMID: 29181388] (Human)
• SH Kim, HJ Choi, WK Yang, JE Lee, JH Cho, IJ Park, S Park, BK Park, M Jin Suppressive Effect of the n-Hexane Extract of Litsea japonica Fruit Flesh on Monosodium-Iodoacetate-Induced Osteoarthritis in Rats Evid Based Complement Alternat Med, 2017-08-22;2017(0):1791403. 2017-08-22 [PMID: 28904551] (Rat)
• X Jiang, JS Hawkins, J Lee, CO Lizama, FL Bos, JP Zape, P Ghatpande, Y Peng, J Louie, G Lagna, AC Zovein, A Hata Let-7 microRNA-dependent control of leukotriene signaling regulates the transition of hematopoietic niche in mice Nat Commun, 2017-07-25;8(1):128. 2017-07-25 [PMID: 28743859] (Mouse)
• K Izawa, A Maehara, M Isobe, Y Yasuda, M Urai, Y Hoshino, K Ueno, T Matsukawa, M Takahashi, A Kaitani, E Shiba, A Takamori, S Uchida, K Uchida, K Maeda, N Nakano, Y Yamanishi, T Oki, D Voehringer, A Roers, S Nakae, J Ishikawa, Y Kinjo, T Shimizu, H Ogawa, K Okumura, T Kitamura, J Kitaura Disrupting ceramide-CD300f interaction prevents septic peritonitis by stimulating neutrophil recruitment Sci Rep, 2017-06-27;7(1):4298. 2017-06-27 [PMID: 28655892] (Mouse)
• CM Low, S Akthar, DF Patel, S Löser, CT Wong, PL Jackson, JE Blalock, SA Hare, CM Lloyd, RJ Snelgrove The development of novel LTA4H modulators to selectively target LTB4 generation Sci Rep, 2017-03-17;7(0):44449. 2017-03-17 [PMID: 28303931] (Human, Mouse)
• SE Pischke, A Gustavsen, HL Orrem, KH Egge, F Courivaud, H Fontenelle, A Despont, AK Bongoni, R Rieben, TI Tønnessen, MA Nunn, H Scott, H Skulstad, A Barratt-Du, TE Mollnes Complement factor 5 blockade reduces porcine myocardial infarction size and improves immediate cardiac function Basic Res. Cardiol, 2017-03-03;112(3):20. 2017-03-03 [PMID: 28258298] (Porcine)
• M Laube, E Amann, U Uhlig, Y Yang, HW Fuchs, M Zemlin, JC Mercier, RF Maier, HD Hummler, S Uhlig, UH Thome Inflammatory Mediators in Tracheal Aspirates of Preterm Infants Participating in a Randomized Trial of Inhaled Nitric Oxide PLoS ONE, 2017-01-03;12(1):e0169352. 2017-01-03 [PMID: 28046032] (Human)
• O'Dwyer C, O'Brien M, Wormald M, White M, Banville N, Hurley K, McCarthy C, McElvaney N, Reeves E The BLT1 Inhibitory Function of alpha-1 Antitrypsin Augmentation Therapy Disrupts Leukotriene B4 Neutrophil Signaling. J Immunol, 2015-09-14;195(8):3628-41. 2015-09-14 [PMID: 26371243] (Human)
• Gounaris E, Heiferman M, Heiferman J, Shrivastav M, Vitello D, Blatner N, Knab L, Phillips J, Cheon E, Grippo P, Khazaie K, Munshi H, Bentrem D Zileuton, 5-lipoxygenase inhibitor, acts as a chemopreventive agent in intestinal polyposis, by modulating polyp and systemic inflammation. PLoS ONE, 2015-03-06;10(3):e0121402. 2015-03-06 [PMID: 25747113] (Human, Mouse)
• Kovacs M, Nemeth T, Jakus Z, Sitaru C, Simon E, Futosi K, Botz B, Helyes Z, Lowell C, Mocsai A The Src family kinases Hck, Fgr, and Lyn are critical for the generation of the in vivo inflammatory environment without a direct role in leukocyte recruitment. J Exp Med, 2014-09-15;211(10):1993-2011. 2014-09-15 [PMID: 25225462] (Mouse)
• Li J, Li F, Wei D, Jia W, Kang J, Stefanovic-Racic M, Dai Y, Zhao A Endogenous omega-3 polyunsaturated fatty acid production confers resistance to obesity, dyslipidemia, and diabetes in mice. Mol Endocrinol, 2014-06-30;28(8):1316-28. 2014-06-30 [PMID: 24978197] (Mouse)
• Schneider B, Behrends J, Hagens K, Harmel N, Shayman J, Schaible U Lysosomal phospholipase A2: a novel player in host immunity to Mycobacterium tuberculosis. Eur J Immunol, 2014-06-11;44(8):2394-404. 2014-06-11 [PMID: 24825529] (Mouse)
• Paige M, Wang K, Burdick M, Park S, Cha J, Jeffery E, Sherman N, Shim Y Role of leukotriene A4 hydrolase aminopeptidase in the pathogenesis of emphysema. J Immunol, 2014-04-25;192(11):5059-68. 2014-04-25 [PMID: 24771855] (Mouse)
• van den Borne P, van der Laan S, Bovens S, Koole D, Kowala M, Michael L, Schoneveld A, van de Weg S, Velema E, de Vries J, de Borst G, Moll F, de Kleijn D, Quax P, Hoefer I, Pasterkamp G Leukotriene B4 levels in human atherosclerotic plaques and abdominal aortic aneurysms. PLoS ONE, 2014-01-27;9(1):e86522. 2014-01-27 [PMID: 24475136] (Human)
• Sapey E, Greenwood H, Walton G, Mann E, Love A, Aaronson N, Insall R, Stockley R, Lord J Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence. Blood, 2013-11-04;123(2):239-48. 2013-11-04 [PMID: 24191150] (Human)
• Molina-Berrios A, Campos-Estrada C, Henriquez N, Faundez M, Torres G, Castillo C, Escanilla S, Kemmerling U, Morello A, Lopez-Munoz R, Maya J Protective role of acetylsalicylic acid in experimental Trypanosoma cruzi infection: evidence of a 15-epi-lipoxin A(4)-mediated effect. PLoS Negl Trop Dis, 2013-04-18;7(4):e2173. 2013-04-18 [PMID: 23638194] (Mouse)
• Dillahunt S, Sargent J, Suzuki R, Proia R, Gilfillan A, Rivera J, Olivera A Usage of sphingosine kinase isoforms in mast cells is species and/or cell type determined. J Immunol, 2013-01-28;190(5):2058-67. 2013-01-28 [PMID: 23359503] (Mouse)
• Auner B, Geiger EV, Henrich D, Lehnert M, Marzi I, Relja B Circulating leukotriene B4 identifies respiratory complications after trauma. Mediators Inflamm., 2012-03-07;2012(0):536156. 2012-03-07 [PMID: 22529525] (Human)
• Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans. J. Immunol., 2011-09-30;187(9):4913-9. 2011-09-30 [PMID: 21964028] (Human, Porcine)
• Bassaganya-Riera J, Guri AJ, Lu P, Climent M, Carbo A, Sobral BW, Horne WT, Lewis SN, Bevan DR, Hontecillas R Abscisic acid regulates inflammation via ligand-binding domain-independent activation of peroxisome proliferator-activated receptor gamma. J. Biol. Chem., 2010-11-18;286(4):2504-16. 2010-11-18 [PMID: 21088297] (Mouse)
• Chou RC, Kim ND, Sadik CD Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis. Immunity, 2010-08-27;33(2):266-78. 2010-08-27 [PMID: 20727790] (Mouse)
• Narasaraju T, Ng HH, Phoon MC MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis. Am. J. Respir. Cell Mol. Biol., 2009-07-17;42(6):732-43. 2009-07-17 [PMID: 19617401] (Mouse)
• Seo KW, Lee SJ, Kim CE Participation of 5-lipoxygenase-derived LTB(4) in 4-hydroxynonenal-enhanced MMP-2 production in vascular smooth muscle cells. Atherosclerosis, 2009-06-18;208(1):56-61. 2009-06-18 [PMID: 19586628] (Mouse)
• Sanchez-Galan E, Gomez-Hernandez A, Vidal C, Martin-Ventura JL, Blanco-Colio LM, Munoz-Garcia B, Ortega L, Egido J, Tuñón J Leukotriene B4 enhances the activity of nuclear factor-kappaB pathway through BLT1 and BLT2 receptors in atherosclerosis. Cardiovasc. Res., 2008-10-13;81(1):216-25. 2008-10-13 [PMID: 18852255] (Human)
• Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma. Thorax, 2008-08-29;63(12):1070-5. 2008-08-29 [PMID: 18757458] (Human)
• Gomez-Hernandez A, Sanchez-Galan E, Ortego M, Martin-Ventura JL, Blanco-Colio LM, Tarin-Vicente N, Jimenez-Nacher JJ, Lopez-Bescos L, Egido J, Tuñón J Effect of intensive atorvastatin therapy on prostaglandin E2 levels and metalloproteinase-9 activity in the plasma of patients with non-ST-elevation acute coronary syndrome. Am. J. Cardiol., 2008-04-22;102(1):12-8. 2008-04-22 [PMID: 18572029] (Human)
• Arias MA, Garcia-Rio F, Alonso-Fernandez A, Hernanz A, Hidalgo R, Martinez-Mateo V, Bartolome S, Rodriguez-Padial L CPAP decreases plasma levels of soluble tumour necrosis factor-alpha receptor 1 in obstructive sleep apnoea. Eur. Respir. J., 2008-05-28;32(4):1009-15. 2008-05-28 [PMID: 18508832] (Human)
• von Bismarck P, Wistadt CF, Klemm K, Winoto-Morbach S, Uhlig U, Schutze S, Adam D, Lachmann B, Uhlig S, Krause MF Improved pulmonary function by acid sphingomyelinase inhibition in a newborn piglet lavage model. Am. J. Respir. Crit. Care Med., 2008-02-28;177(11):1233-41. 2008-02-28 [PMID: 18310483] (Porcine)