| Dilutions |
|
| Application Notes | Each tissue or cell lysate
is prepared in 1x sample buffer at a concentration
ideal for most SDS-PAGE applications.
One vial of this Lysate is
sufficient to run 10 lanes (10 ug/lane) of protein. All
tissues are extracted from adult mice. |
| Storage | Store at -80C in the dark. Avoid freeze-thaw cycles. |
| Buffer | 1 x SDS sample buffer:
60 mM Tris-Hcl (pH 6.8)
10 mM EDTA
2 % SDS
0.03% Bromophenol Blue
10% glycerol
0.3% Beta-mercaptoethanol |
| Concentration | 1 mg/ml |
|
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