Bovine Liver Whole Tissue Lysate (Adult Whole Normal)

Product Discontinued

Bovine Liver Whole Tissue Lysate (Adult Whole Normal) Summary

• Description: We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
• Specificity: Total Protein - Bovine Normal Tissue: Liver

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Reactivity Notes

Cross reacts with Bovine.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol and Sodium deoxycholate
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Digestive/Waste
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Alternate Names for Bovine Liver Whole Tissue Lysate (Adult Whole Normal)

• Liver lysate
• liver lysates
• Liver

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Liver Lysate (NB820-59149). (Showing 1 - 1 of 1 FAQ).

1. I need a positive control for Western Blot to make sure that my primary antibody is ok. I am a novice in proteomics so I have to ask, can I use tissue lysate products for this purpose? I am trying to detect a multipass membrane protein from bovine liver but I haven't been successful so far. I have found a product from Novus Biologicals website, a whole normal liver lysate for bovine liver (NB820-59149, 1 mg). Does this whole lysate include proteins from different cellular compartments including membrane bound proteins? Can I use this product as a positive control in WB? How quickly I could get this product delivered?
   • If the protein of interest is located in liver samples without any necessary induction through special treatments, then it should be present in the whole normal liver lysate from bovine. Depending on if your antibody can detect the native protein, or denaturing conditions must be used, could also be a reason for difficulties detecting.