Bovine Liver Whole Tissue Lysate (Adult Whole Normal)

Images

 
There are currently no images for Liver Lysate (NB820-59149).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Bovine Liver Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Bovine Normal Tissue: Liver

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Reactivity Notes

Cross reacts with Bovine.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol and Sodium deoxycholate
Concentration
5.0 mg/ml

Lysate Details for Liver

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Alternate Names for Bovine Liver Whole Tissue Lysate (Adult Whole Normal)

  • Liver lysate
  • liver lysates
  • Liver

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Liver Lysate (NB820-59149) (0)

There are no publications for Liver Lysate (NB820-59149).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Liver Lysate (NB820-59149) (0)

There are no reviews for Liver Lysate (NB820-59149). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Liver Lysate (NB820-59149). (Showing 1 - 1 of 1 FAQ).

  1. I need a positive control for Western Blot to make sure that my primary antibody is ok. I am a novice in proteomics so I have to ask, can I use tissue lysate products for this purpose? I am trying to detect a multipass membrane protein from bovine liver but I haven't been successful so far. I have found a product from Novus Biologicals website, a whole normal liver lysate for bovine liver (NB820-59149, 1 mg). Does this whole lysate include proteins from different cellular compartments including membrane bound proteins? Can I use this product as a positive control in WB? How quickly I could get this product delivered?
    • If the protein of interest is located in liver samples without any necessary induction through special treatments, then it should be present in the whole normal liver lysate from bovine. Depending on if your antibody can detect the native protein, or denaturing conditions must be used, could also be a reason for difficulties detecting.

Additional Liver Products

Blogs on Liver.

Signalling Advances in Adiponectin Antibody Research
Adiponectin is an adipocytokine protein that positively regulates metabolism of lipids and glucose by suppressing glucose production from the liver, stimulating insulin sensitivity, and increasing the rate of fatty acid oxidation and glucose uptake. I...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Bovine Liver Whole Tissue Lysate (Adult Whole Normal) and receive a gift card or discount.