Rat Liver Whole Tissue Lysate (Adult Whole Normal)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB800-PC23
    • Availability
      Product Discontinued

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Rat Liver Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Normal Rat Liver whole cell extract lysate

Applications/Dilutions

Application Notes
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents. Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X SDS-PAGE Sample Buffer [62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8], 10% (v/v) Glycerol
Preservative
No Preservative
Concentration
1.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Alternate Names for Rat Liver Whole Tissue Lysate (Adult Whole Normal)

  • Liver lysate
  • liver lysates
  • Liver

Background

This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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