Lipid A Antibody (26-5)

• Reactivity: Ba
• Applications: ELISA
• Clone: 26-5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Lipid A Antibody (26-5) Summary

• Immunogen: The monoclonal antibody 26-5 recognizes lipid A.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
• Application Notes: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Lipid A Antibody (26-5)

• Lipid A Lipopolysaccharide

Background

The monoclonal antibody 26-5 recognizes lipid A. The lipid A domain of lipopolysaccharide (LPS) is a unique, glucosamine-based phospholipid that makes up the outer monolayer of the outer membrane of most gram-negative bacteria.
Lipid A is responsible for the endotoxic activities of LPS, and a heteropolysaccharide. The heteropolysaccharide generally consists of an oligosaccharide termed the core region. This core region is in some bacterial families composed of repeating oligosaccharide units, the so-called O chain. The lipid A moiety of LPS is detected by the TLR4/MD2 receptor of the mammalian innate immune system. Picomolar levels of lipid A induce macrophages to synthesize potent mediators of inflammation, such as TNF-alpha and IL-1beta. Furthermore, lipid A activates the production of costimulatory molecules required for adaptive immunity and stimulates tissue factor production in mononuclear and endothelial cells. All these events are desirable for clearing local infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.