Lewis Y Antibody (A70-A/A9)

Immunohistochemistry-Paraffin: Lewis Y Antibody (A70-A/A9) [NBP2-50073] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Lewis Y Monoclonal Antibody (A70-A/A9).

• Reactivity: Hu
• Applications: Flow, IHC, IHC-P, ICC/IF
• Clone: A70-A/A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Lewis Y Antibody (A70-A/A9) Summary

• Immunogen: Live Ls174T cells (human colon carcinoma cell line)
• Epitope: (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-
• Localization: Cell membrane, cytoplasmic and extracellular
• Marker: Tumor Marker
• Specificity: This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54561)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Lewis Y Antibody (A70-A/A9)

• IPCA-2
• IPCA6
• IPCA-6
• IPCA-8
• PCANAP2
• PCANAP6
• PCANAP8
• Prostate cancer-associated protein 6
• PRST
• Solute carrier family 45 member 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.