Lewis Y Antibody (A70-A/A9)

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Immunohistochemistry-Paraffin: Lewis Y Antibody (A70-A/A9) [NBP2-50073] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Lewis Y Monoclonal Antibody (A70-A/A9).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC-P, ICC/IF
Clone
A70-A/A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Lewis Y Antibody (A70-A/A9) Summary

Immunogen
Live Ls174T cells (human colon carcinoma cell line)
Localization
Cell membrane, cytoplasmic and extracellular
Marker
Tumor Marker
Specificity
This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1ug/million cells
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunofluorescence 0.5-1ug/ml
Application Notes
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-54561AF350
Alexa Fluor 405 NBP2-54561AF405
Alexa Fluor 488 NBP2-54561AF488
Alexa Fluor 532 NBP2-54561AF532
Alexa Fluor 594 NBP2-54561AF594
Alexa Fluor 647 NBP2-54561AF647
Alexa Fluor 700 NBP2-54561AF700
Alexa Fluor 750 NBP2-54561AF750
Allophycocyanin NBP2-54561APC
Allophycocyanin/Cy7 NBP2-54561APCCY7
Biotin NBP2-54561B
DyLight 350 NBP2-54561UV
DyLight 405 NBP2-54561V
DyLight 550 NBP2-54561R
DyLight 594 NBP2-54561DL594
DyLight 650 NBP2-54561C
DyLight 680 NBP2-54561FR
DyLight 755 NBP2-54561IR
FITC NBP2-54561F
HRP NBP2-54561H
Janelia Fluor 549 NBP2-54561JF549
Janelia Fluor 646 NBP2-54561JF646
PE NBP2-54561PE
PE/Atto594 NBP2-54561PEATT594
PE/Cy5.5 NBP2-54561PECY55
PE/Cy7 NBP2-54561PECY7
PerCP NBP2-54561PCP

Additional Lewis Y Products

Array NBP2-50073

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