Goat anti-Human Lambda Light Chain Secondary Antibody

• Reactivity: Hu
• Applications: WB, DB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Goat anti-Human Lambda Light Chain Secondary Antibody Summary

• Immunogen: A pool of purified Bence Jones lambda proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of human serum or plasma.
• Clonality: Polyclonal
• Host: Goat
• Gene: IGLC1
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Western Blot
• Application Notes: Indirect immunofluorescence

Reactivity Notes

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ion exchange chromatography
• Reconstitution Instructions: Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Notes

The lyophilized product is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Alternate Names for Goat anti-Human Lambda Light Chain Secondary Antibody

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGLC1