Goat anti-Human Lambda Light Chain Secondary Antibody [HRP]

Western Blot: Goat anti-Human Lambda Light Chain Secondary Antibody [HRP] [NBP1-73715] - Lane 1: Human Lambda. Lane 2: None. Load: 50 ng per lane. Primary Antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 kDa for Human Lambda. Other band(s): none.

Product Discontinued

Goat anti-Human Lambda Light Chain Secondary Antibody [HRP] Summary

• Immunogen: Goat anti-Human Lambda Light Chain Secondary Antibody [HRP] was produced by repeated immunization with human lambda light chain in goat.
• Specificity: Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGLC1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Western Blot 1:1000 - 1:10000
• Application Notes: This secondary antibody has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:16,000 to 1:64,000 is suggested for this product.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Thimerosal
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent).

Notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Goat anti-Human Lambda Light Chain Secondary Antibody [HRP]

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Lambda Light Chain Antibody (NBP1-73715)(1)

Publication using NBP1-73715

• Luo G, Yogeshwar S, Lin L Et al. T cell reactivity to regulatory factor X4 in type 1 narcolepsy Sci Rep 2021-04-10 [PMID: 33837283]
  
  Details:
  Citation using the HRP version of this antibody.

Review for Lambda Light Chain Antibody (NBP1-73715) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 07/29/2019

Summary

• Application: Western Blot
• Sample Tested: Human IgG
• Species: Human
• Lot: 39168

Bioinformatics

• Gene Symbol: IGLC1