Goat anti-Human Lambda Light Chain Secondary Antibody [Janelia Fluor 646]


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Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Janelia Fluor 646

Goat anti-Human Lambda Light Chain Secondary Antibody [Janelia Fluor 646] Summary

Goat anti-Human Lambda Light Chain Secondary Antibody was produced by repeated immunization with Human lambda light chain molecule in goat.
Specificity was confirmed by ELISA.


  • ELISA 1:4000 - 1:20000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:500 - 1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide


Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Goat anti-Human Lambda Light Chain Secondary Antibody [Janelia Fluor 646]

  • IGLC
  • immunoglobulin lambda constant group


All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Gene Symbol IGLC1