Rabbit anti-Mouse Lambda Light Chain Secondary Antibody [Alkaline Phosphatase]

Product Discontinued

Rabbit anti-Mouse Lambda Light Chain Secondary Antibody [Alkaline Phosphatase] Summary

• Description: Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), and anti-Rabbit Serum
• Immunogen: Mouse lambda light chain
• Specificity: Specificity was confirmed by ELISA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGLC1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Immunohistochemistry 1:200 - 1:1000
  • Western Blot 1:500 - 1:2500
• Application Notes: This product has been tested by ELISA and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Rabbit anti-Mouse Lambda Light Chain Secondary Antibody [Alkaline Phosphatase]

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGLC1