Sheep anti-Human Lambda Light Chain Secondary Antibody

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Sheep anti-Human Lambda Light Chain Secondary Antibody Summary

• Immunogen: Human Lambda Free Light Chain
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with lambda free light chains. No was detected against kappa light chain or other serum proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IGLC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:30000 (Primary) 1:100-1:500 (For Coating Plates)
  • Immunocytochemistry/ Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin 1:200-1:2000
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sheep anti-Human Lambda Light Chain Secondary Antibody

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGLC1