Goat anti-Human Lambda Light Chain Secondary Antibody [HRP]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Goat anti-Human Lambda Light Chain Secondary Antibody [HRP] Summary

Immunogen
Human Lambda Light Chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with lambda light chains common to all human immunoglobulins. No antibody was detected against kappa light chain or non-immunoglobulin serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGLC2
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:20000
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
Readout System
Publications
Read Publication using NB7471.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.1% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Human Lambda Light Chain Secondary Antibody [HRP]

  • IGLC
  • immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30, the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain are present in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lambda Light Chain Antibody (NB7471)(1)

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IGLC2