Lambda Light Chain Antibody (RM110) [Biotin]

Western Blot: Rabbit anti-Mouse Lambda Light Chain Secondary Antibody (RM110) [Biotin] [NBP2-62002B] - Western blot of nonreduced(-) and reduced(+) mouse IgG1(kappa) and IgG1(lambda) (20ng/lane), using0.2ug/mL of NBP2-62002B. This antibody reacts to nonreduced IgG1(Lambda).

ELISA: Rabbit anti-Mouse Lambda Light Chain Secondary Antibody (RM110) [Biotin] [NBP2-62002B] - ELISA of mouse immunoglobulins shows NBP2-62002B reacts to the lambda light chain of mouse immuno-globulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG. The plate was coated with different immuno-globulins. NBP2-62002B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA: Rabbit anti-Mouse Lambda Light Chain Secondary Antibody (RM110) [Biotin] [NBP2-62002B] - A titer ELISA of mouse IgG1 lambda. The plate was coated with different amounts of mouse IgG1 lambda. A serial dilution of NBP2-62002B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

• Reactivity: Mu
• Applications: WB, ELISA
• Clone: RM110
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Biotin
• Concentration: 1 mg/ml

Lambda Light Chain Antibody (RM110) [Biotin] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse IgM Lambda
• Specificity: This antibody reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGLC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.005 ug/ml - 0.2 ug/ml
  • Western Blot 0.1 - 0.5 ug/mL
• Application Notes: This primary antibody can also be used as a secondary antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, 1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Lambda Light Chain Antibody (RM110) [Biotin]

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGLC1