Japanese Encephalitis virus NS1 Antibody (995010) [Unconjugated]

Western blot shows recombinant Japanese Encephalitis virus NS1 (rvJEV NS1). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB10006) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Japanese Encephalitis virus NS1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB
• Clone: 995010
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Japanese Encephalitis virus NS1 Antibody (995010) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived Japanese Encephalitis virus NS1
  Thr796-Ala1146
  Accession # P32886
• Specificity: Detects Japanese Encephalitis virus NS1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Japanese Encephalitis virus NS1 Antibody (995010) [Unconjugated]

• Japanese Encephalitis virus NS1
• Japanese Encephalitis
• JE virus
• JEV
• JEVgp1
• JEV-NS1

Background

Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

1. Nain, M. et al. (2016) Rev. Med. Virol. 26:129.
2. Rastogi, M. et al. (2016) Virol. J. 13:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Japanese Encephalitis virus NS1 Antibody (MAB10006)(1)

Publication using MAB10006

• Y Park, AR Kim, YH Hwang, H Yang, JW Lee, MY Kim, HS Kim, GT Chung, JS Yoo, YJ Kim, D Kim, H Won Comparison of plaque reduction and focus reduction neutralization tests for the measurement of neutralizing antibody titers against japanese encephalitis virus Journal of virological methods, 2022-05-10;306(0):114540. 2022-05-10 [PMID: 35550887] (ICC, African Green Monkey)

Bioinformatics

• Uniprot:
  • P32886()