INSTA-Blot Multi-species Skeletal Muscle, Heart and Kidney Tissues

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SDS-Page: INSTA-Blot Multi-species Skeletal Muscle, Heart and Kidney Tissues [NBP2-30116] - Approximately 20 ug per lane of tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido ...read more

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Summary

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INSTA-Blot Multi-species Skeletal Muscle, Heart and Kidney Tissues Summary

Description
Ready-to-use PVDF membrane with 11 human, mouse and rat tissue lysates (20 ug total protein per lane).
Background
INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Multi-species Skeletal Muscle, Heart, Kidney Tissues NBP2-30116 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS...-PAGE and electro-blotting steps. With INSTA-Blot™ immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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Preparation
Method
INSTA-Blot™ Multi-species Skeletal Muscle, Heart, Kidney Tissue Lysate NBP2-30116 contains denatured proteins from tissue lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4- 20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot ™ is provided ready-to-use in your western blot protocol.

Applications/Dilutions

Application Notes
Human tissues are from donors with no known disease. Mouse tissues are from Balb/c mice. Rat tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% b-mercaptoethanol).

Packaging, Storage & Formulations

Storage
Store at room temperature.

Notes

The molecular weights for the marker used with this product are: 180, 116, 97, 66, 44, 29, 18, 14, 6.

Alternate Names for INSTA-Blot Multi-species Skeletal Muscle, Heart and Kidney Tissues

  • INSTA Blot Multi-species Skeletal Muscle, Heart, Kidney Tissues
  • INSTABlot Multi-species Skeletal Muscle, Heart, Kidney Tissues
  • INSTA-Blot Multi-species Skeletal Muscle, Heart, Kidney

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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