Influenza A Virus M2 Antibody (14C2)

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Immunocytochemistry/ Immunofluorescence: Influenza A Virus M2 Antibody (14C2) [NB100-2073] - Analysis of MDCK cells

Product Details

Summary
Reactivity Hu, V-Vi, XpSpecies Glossary
Applications WB, Flow, ICC/IF, IP, B/N
Clone
14C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Influenza A Virus M2 Antibody (14C2) Summary

Immunogen
M2 protein from A/WSN/33-infected CV1 cell lysate.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 10 ug/ml
  • Block/Neutralize
Application Notes
Blocking usage reported in literature (PMID: 7571410). WB: Detects an approx. 15 kDa protein representing the M2 protein. It has demonstrated the ability to restrict the replication of some strains of influenza A virus by binding to the extracellular N-Terminal domain. May be useful in IHC.
Publications
Read Publications using
NB100-2073 in the following applications:

  • 2 publications
  • WB
    3 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23049700).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Background

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Influenza A Virus M2 Antibody (NB100-2073)(7)

We have publications tested in 3 confirmed species: Human, Virus, Xenopus.

We have publications tested in 2 applications: FLOW, WB.


Filter By Application
FLOW
(2)
WB
(3)
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Filter By Species
Human
(2)
Virus
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-2073 Applications Species
Schmid ET, Pang IK, Carrera Silva EA et al. AXL receptor tyrosine kinase is required for T cell priming and antiviral immunity. Elife 2016 Jun 28 [PMID: 27350258] (FLOW) FLOW
Londino JD, Lazrak A, Noah JW et al. Influenza virus M2 targets cystic fibrosis transmembrane conductance regulator for lysosomal degradation during viral infection. FASEB J. 2015 Mar 20 [PMID: 25795456] (WB, Human) WB Human
Pang IK, Pillai PS, Iwasaki A et al. Efficient influenza A virus replication in the respiratory tract requires signals from TLR7 and RIG-I. Proc Natl Acad Sci U S A 2013 Aug 5 [PMID: 23918369] (FLOW, Virus) FLOW Virus
Londino JD, Lazrak A, Jurkuvenaite A et al. Influenza Matrix Protein 2 Alters CFTR Expression and Function through its Ion Channel Activity Am J Physiol Lung Cell Mol Physiol 2013 Mar 1 [PMID: 23457187] (WB, Xenopus, Human) WB Xenopus, Human
Lin SC, Lin YF, Chong P, Wu SC. Broader Neutralizing Antibodies against H5N1 Viruses Using Prime-Boost Immunization of Hyperglycosylated Hemagglutinin DNA and Virus-Like Particles PLoS One 2012 [PMID: 22720032] (WB) WB
Wei H-J, Chang W, Lin S-C et al. Fabrication of influenza virus-like particles using M2 fusion proteins for imaging single viruses and designing vaccines. Vaccine. 2011 Jun 7. [PMID: 21651946]
Pan YS, Wei HJ, Chang CC et al. Construction and characterization of insect cell-derived influenza VLP: cell binding, fusion, and EGFP incorporation. J Biomed Biotechnol 2010 [PMID: 21197092]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Influenza A Virus M2 Antibody (NB100-2073). (Showing 1 - 1 of 1 FAQs).

  1. Could you cite this antibody's purity % as well as the means by which purity was determined?
    • This particular antibody was protein G purified from ascites. We unfortunately do not measure the purity of our antibodies, but it is expected to be above 95%.

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