Influenza A H5N1 Hemagglutinin Antibody (2B7) - (A/Vietnam/HN31242/2007) - BSA Free

Western Blot: Influenza A H5N1 Hemagglutinin Antibody (2B7) - (A/Vietnam/HN31242/2007) - BSA Free [NBP2-22605] - Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Influenza A H5N1 Hemagglutinin/HA1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: H7N9-HA1(19-339aa) (A/Anhui/1-BALF_RG45/2013)Lane 2.: H7N9-HA1(1-523aa)(A/Shanghai/JS01/2013)Lane 3.: H5N1-HA1(17-338aa) (A/Vietnam/HN31242/2007)

Western Blot: Influenza A H5N1 Hemagglutinin Antibody (2B7) - (A/Vietnam/HN31242/2007) - BSA Free [NBP2-22605] - Influenza A H5N1 Hemagglutinin recombinant protein (50ng) resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Influenza A H5N1 Hemagglutinin antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ELISA: Influenza A H5N1 Hemagglutinin Antibody (2B7) - (A/Vietnam/HN31242/2007) - BSA Free [NBP2-22605] - Analysis using ELISA.

• Reactivity: V
• Applications: WB, ELISA
• Clone: 2B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Influenza A H5N1 Hemagglutinin Antibody (2B7) - (A/Vietnam/HN31242/2007) - BSA Free Summary

• Description: Novus Biologicals Mouse Influenza A H5N1 Hemagglutinin Antibody (2B7) - (A/Vietnam/HN31242/2007) - BSA Free (NBP2-22605) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant Influenza A H5N1 Hemagglutinin (17-338aa) purified from Baculovirus
• Specificity: Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Background

H5N1 is a subtype of the species Influenza A virus of the Influenzavirus A genus of the Orthomyxoviridae family. consists of single-stranded eight-segment negative-sense genomic RNAs, helical viral ribonucleoprotein (RNP) complexes (RNA segments NP, PB2, PB1 and PA), three viral envelope proteins (hemagglutinin [HA], neuraminidase [NA], and M2 ion channel), and a maxtir (M1) protein. Influenza A viruses are further classified into 16 HA (H1-H16) and 9 NA (N1-N9) serotypes based on the antigenic characteristics of HA and NA envelope glycoproteins. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.