Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) - Azide and BSA Free

Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Influenza A H1N1 Nucleoprotein antibody [GT1236] detects Influenza A H1N1 Nucleoprotein by Western blot analysis. A. 5 ug DF1 whole cell lysate/extract B. 5 ug whole cell lysate/extract of WSN virus infected DF1 cells (10 hr) 7.5 % SDS-PAGE Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) dilution: 1:5000

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Influenza A H1N1 Nucleoprotein antibody [GT1236] detects Influenza A H1N1 Nucleoprotein by immunofluorescent analysis. Sample: DF1 cells infected with Influenza A virus (WSN) . Green: Influenza A H1N1 Nucleoprotein stained by Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) diluted at 1:500. Blue: Hoechst 33342 staining.

Sandwich ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) as capture antibody at concentration of 5 ug/mL and Influenza A H1N1 Nucleoprotein antibody (NBP2-16965) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Sandwich ELISA detection of recombinant full-length Influenza A H1N1 Nucleoprotein, DDDDK tag using Influenza A H1N1 Nucleoprotein antibody (NBP2-16965) as capture antibody at concentration of 5 ug/mL and Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA: Influenza A H1N1 Nucleoprotein Antibody (GT1236) [NBP3-13493] - Sandwich ELISA detection of recombinant full-length Influenza A H1N1 Nucleoprotein, DDDDK tag using Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) as capture antibody at concentration of 5 ug/mL and Influenza A H1N1 Nucleoprotein antibody (NBP2-16965) as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Western Blot: Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) [NBP3-13493] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A H1N1 Nucleoprotein antibody [GT1236] (NBP3-13493) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag using antibodies as below. Capture: Influenza A virus Nucleoprotein antibody [HL4098] (5 ug/mL) Detection: Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) (1 ug/mL)

Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag using antibodies as below. Capture: Influenza A virus Nucleoprotein antibody [HL1103] (5 ug/mL) Detection: Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) (1 ug/mL)

Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag using antibodies as below. Capture: Influenza A virus Nucleoprotein antibody [HL1089] (5 ug/mL) Detection: Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) (1 ug/mL)

Sandwich ELISA detection of recombinant Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag using antibodies as below. Capture: Influenza A virus Nucleoprotein antibody [HL1098] (5 ug/mL) Detection: Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) (1 ug/mL)

Influenza A virus Nucleoprotein antibody [GT1236] detects Influenza A virus Nucleoprotein protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody [GT1236] (NBP3-13493) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: GT1236
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Influenza A H1N1 Nucleoprotein Antibody (GT1236) - (A/WSN/1933) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein of Influenza A H1N1 Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.
• Specificity: Influenza A H1N1 Nucleoprotein. (A/WSN/1933)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.