Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933)

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933) [NB100-2073] - Analysis of MDCK cells

• Reactivity: V
• Applications: WB, Flow, ICC/IF, IP, B/N
• Clone: 14C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933) Summary

• Immunogen: M2 protein from A/WSN/33-infected CV1 cell lysate.
• Specificity: Influenza A H1N1 M2 (A/WSN/1933)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunoprecipitation 10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: Blocking usage reported in literature (PMID: 7571410). WB: Detects an approx. 15 kDa protein representing the M2 protein. It has demonstrated the ability to restrict the replication of some strains of influenza A virus by binding to the extracellular N-Terminal domain. May be useful in IHC.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933)

• Influenza M2

Background

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Influenza A H1N1 M2 Antibody (NB100-2073)(8)

Publications using NB100-2073

• Tsai M, Osman W, Adair J et al. The E3 ligase subunit FBXO45 binds the interferon-lambda receptor and promotes its degradation during influenza virus infection The Journal of biological chemistry 2022-11-12 [PMID: 36379255] (WB)
• Schmid ET, Pang IK, Carrera Silva EA et al. AXL receptor tyrosine kinase is required for T cell priming and antiviral immunity. Elife 2016-06-28 [PMID: 27350258] (FLOW)
• Londino JD, Lazrak A, Noah JW et al. Influenza virus M2 targets cystic fibrosis transmembrane conductance regulator for lysosomal degradation during viral infection. FASEB J. 2015-03-20 [PMID: 25795456] (WB, Human)
• Pang IK, Pillai PS, Iwasaki A et al. Efficient influenza A virus replication in the respiratory tract requires signals from TLR7 and RIG-I. Proc Natl Acad Sci U S A 2013-08-05 [PMID: 23918369] (FLOW, Virus)
• Londino JD, Lazrak A, Jurkuvenaite A et al. Influenza Matrix Protein 2 Alters CFTR Expression and Function through its Ion Channel Activity Am J Physiol Lung Cell Mol Physiol 2013-03-01 [PMID: 23457187] (WB, Human, Xenopus)
• Lin SC, Lin YF, Chong P et al. Broader neutralizing antibodies against H5N1 viruses using prime-boost immunization of hyperglycosylated hemagglutinin DNA and virus-like particles. PLoS One. 2012-01-01 [PMID: 22720032] (WB)
  
  Details:
  This citation used the HRP form of this antibody.
• Wei H-J, Chang W, Lin S-C et al. Fabrication of influenza virus-like particles using M2 fusion proteins for imaging single viruses and designing vaccines. Vaccine. 2011-06-07 [PMID: 21651946]
• Pan YS, Wei HJ, Chang CC et al. Construction and characterization of insect cell-derived influenza VLP: cell binding, fusion, and EGFP incorporation. J Biomed Biotechnol 2010-01-01 [PMID: 21197092]

FAQs for Influenza A H1N1 M2 Antibody (NB100-2073). (Showing 1 - 1 of 1 FAQs).

1. Could you cite this antibody's purity % as well as the means by which purity was determined?
   • This particular antibody was protein G purified from ascites. We unfortunately do not measure the purity of our antibodies, but it is expected to be above 95%.