Mouse IgG2b Isotype Control (73009) [Unconjugated]

• Reactivity: Mu
• Applications: Ctrl
• Clone: 73009
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Mouse IgG2b Isotype Control (73009) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant protein with a C-terminal E-tag (aa sequence GAPVPYPDPLEPR)
• Specificity: Recognizes the E-tag peptide. No background staining was detected by immunohistochemistry on a variety of tissues.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Control 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IgG2b Isotype Control (73009) [Unconjugated]

• IgG2B

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2b Isotype Control (MAB0042)(9)

Publications using MAB0042

• CM Gromisch, GLA Tan, KA Pasion, AM Moran, MS Gromisch, MW Grinstaff, FJ Carr, VLM Herrera, N Ruiz-Opazo Humanized anti-DEspR IgG4S228P antibody increases overall survival in a pancreatic cancer stem cell-xenograft peritoneal carcinomatosis ratnu/nu model BMC Cancer, 2021-04-14;21(1):407. 2021-04-14 [PMID: 33853558] (Flow Cytometry, Human)
• Olga Varea, Jordi Duran, Mònica Aguilera, Neus Prats, Joan J. Guinovart Suppression of glycogen synthesis as a treatment for Lafora disease: Establishing the window of opportunity. Neurobiology of Disease 2021-01-01 [PMID: 33171226]
• Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis E, Bjorkstrom N In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity. PLoS ONE, 2014-08-20;9(8):e105375. 2014-08-20 [PMID: 25141347] (Isotype Control, Human)
• Jiwakanon Jatesada, Persson Elisabeth, Dalin Anne-Marie Seminal plasma did not influence the presence of transforming growth factor-beta 1, interleukine-10 and interleukin-6 in porcine follicles shortly after insemination Acta Veterinaria Scandinavica 2013-12-01 [PMID: 24020676]
• Rossing K, Novak N, Mommert S, Pfab F, Gehring M, Wedi B, Kapp A, Raap U Brain-derived neurotrophic factor is increased in serum and skin levels of patients with chronic spontaneous urticaria. Clin. Exp. Allergy, 2011-06-16;41(10):1392-1399. 2011-06-16 [PMID: 21676041] (Control, Human)
• Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH S100A11, an dual mediator for growth regulation of human keratinocytes. Mol. Biol. Cell, 2007-10-31;19(1):78-85. 2007-10-31 [PMID: 17978094] (Control, Human)
• Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-30;293(1):L142-50. 2007-03-30 [PMID: 17400600] (Control, Mouse)
• Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses. J. Virol., 2007-03-21;81(11):5882-92. 2007-03-21 [PMID: 17376924] (Control, Human)
• Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro. Am. J. Pathol., 2006-12-01;169(6):2094-103. 2006-12-01 [PMID: 17148672] (Control, Human)