Mouse IgG2b Isotype Control (133303) [Allophycocyanin]

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human TRAIL R2/TNFRSF10B APC-conjugated Monoclonal Antibody (Catalog # FAB6311A, filled histogram) or Mouse IgG APC-conjugated Isotype Control Antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Ctrl
• Clone: 133303
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Mouse IgG2b Isotype Control (133303) [Allophycocyanin] Summary

• Immunogen: KLH
• Specificity: Selected for use as mouse IgG_2B isotype control for flow cytometry applications.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Control 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IgG2b Isotype Control (133303) [Allophycocyanin]

• IgG2B

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2b Isotype Control (IC0041A)(21)

Publications using IC0041A

• Francisco Raúl RA贚 BORZONE, María Belén BEL蒒 GIORELLO, Leandro Marcelo MARCELO MARTINEZ, María Cecilia CECILIA SANMARTIN, LEONARDO FELDMAN, FEDERICO DIMASE et al. Senescent mesenchymal stem/stromal cells in pre-metastatic bone marrow of untreated advanced breast cancer patients Oncology Research 2023-05-24 [PMID: 37305388]
• Linda Hofmann, Valentin Medyany, Jasmin Ezić, Ramin Lotfi, Beate Niesler, Ralph Röth et al. Cargo and Functional Profile of Saliva-Derived Exosomes Reveal Biomarkers Specific for Head and Neck Cancer Frontiers in Medicine 2022-07-11 [PMID: 35899209]
• JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus Cellular Signalling, 2022-05-21;96(0):110360. 2022-05-21 [PMID: 35609807] (Flow Cytometry, Human)
• Sofie Hagelund, Anna Trauzold Impact of Extracellular pH on Apoptotic and Non-Apoptotic TRAIL-Induced Signaling in Pancreatic Ductal Adenocarcinoma Cells Frontiers in Cell and Developmental Biology 2022-02-24 [PMID: 35281089]
• Richard E. Beatson, Ana C. Parente-Pereira, Leena Halim, Domenico Cozzetto, Caroline Hull, Lynsey M. Whilding et al. TGF-beta 1 potentiates V gamma 9Vδ2 T cell adoptive immunotherapy of cancer Cell Reports Medicine 2021-12-21 [PMID: 35028614]
• MN Theodoraki, S Laban, EK Jackson, R Lotfi, PJ Schuler, C Brunner, TK Hoffmann, TL Whiteside, L Hofmann Changes in circulating exosome molecular profiles following surgery/(chemo)radiotherapy: early detection of response in head and neck cancer patients British Journal of Cancer, 2021-10-12;0(0):. 2021-10-12 [PMID: 34642463] (Flow Cytometry Control, Human)
• AM Farhat, AC Weiner, C Posner, ZS Kim, B Orcutt-Jah, SM Carlson, AS Meyer Modeling cell-specific dynamics and regulation of the common gamma chain cytokines Cell Reports, 2021-04-27;35(4):109044. 2021-04-27 [PMID: 33910015] (Flow Cytometry Control, Mouse)
• Kodandaram Pillarisetti, Suzanne Edavettal, Mark Mendonça, Yingzhe Li, Mark Tornetta, Alexander Babich et al. A T-cell–redirecting bispecific G-protein–coupled receptor class 5 member D x CD3 antibody to treat multiple myeloma Blood 2020-04-09 [PMID: 32040549]
• U Mert, A Adawy, E Scharff, P Teichmann, A Willms, V Haselmann, C Colmorgen, J Lemke, S von Karste, J Fritsch, A Trauzold TRAIL Induces Nuclear Translocation and Chromatin Localization of TRAIL Death Receptors Cancers (Basel), 2019-08-14;11(8):. 2019-08-14 [PMID: 31416165] (Flow Cytometry, Human)
• A Willms, H Schittek, S Rahn, J Sosna, U Mert, D Adam, A Trauzold Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells PLoS ONE, 2019-04-04;14(4):e0214847. 2019-04-04 [PMID: 30947287] (Flow Cytometry, Human)
• FJ Zapatero-B, E Dietzel, O Dolnik, K Döhner, R Costa, B Hertel, B Veselkova, J Kirui, A Klintworth, MP Manns, S Pöhlmann, T Pietschman, T Krey, S Ciesek, G Gerold, B Sodeik, S Becker, T von Hahn Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors Viruses, 2019-03-19;11(3):. 3/19/2019 [PMID: 30893855]
• AJ Parry, M Hoare, D Bihary, R Hänsel-Her, S Smith, K Tomimatsu, E Mannion, A Smith, P D'Santos, IA Russell, S Balasubram, H Kimura, SA Samarajiwa, M Narita NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence Nat Commun, 2018-05-09;9(1):1840. 2018-05-09 [PMID: 29743479] (Flow Cytometry, Human)
• LV Ageeva, VY Sysoeva, PA Tyurin-Kuz, GV Sharonov, DT Dyikanov, DV Stambolsky, NI Kalinina Data supporting that adipose-derived mesenchymal stem/stromal cells express angiotensin II receptors in situ and in vitro Data Brief, 2017-11-21;16(0):327-333. 2017-11-21 [PMID: 29234689] (Flow Cytometry, Human)
• VY Sysoeva, LV Ageeva, PA Tyurin-Kuz, GV Sharonov, DT Dyikanov, NI Kalinina, VA Tkachuk Local angiotensin II promotes adipogenic differentiation of human adipose tissue mesenchymal stem cells through type 2 angiotensin receptor Stem Cell Res, 2017-11-03;25(0):115-122. 2017-11-03 [PMID: 29127873] (Flow Cytometry Control, Human)
• Anchana Rathinasamy, Christoph Domschke, Yingzi Ge, Hans-Henning Böhm, Steffen Dettling, David Jansen et al. Tumor specific regulatory T cells in the bone marrow of breast cancer patients selectively upregulate the emigration receptor S1P1 Cancer Immunology, Immunotherapy 2017-05-01 [PMID: 28224210]
• Shan Lang, Libing Li, Xuning Wang, Junping Sun, Xinying Xue, Yongjiu Xiao et al. CXCL10/IP-10 Neutralization Can Ameliorate Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome in Rats PLOS ONE 2017-01-03 [PMID: 28046003]
• Roach K, Feghali-Bostwick C, Amrani Y, Bradding P Lipoxin A4 Attenuates Constitutive and TGF-beta1-Dependent Profibrotic Activity in Human Lung Myofibroblasts. J Immunol, 2015-08-14;195(6):2852-60. 2015-08-14 [PMID: 26276873] (Flow Cytometry, Human)
• Daniela Schilling, Annett Kühnel, Fabian Tetzlaff, Sarah Konrad, Gabriele Multhoff NZ28-induced inhibition of HSF1, SP1 and NF-kappa B triggers the loss of the natural killer cell-activating ligands MICA/B on human tumor cells Cancer Immunology, Immunotherapy 2015-05-01 [PMID: 25854583]
• Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin. Immunology, 2009-10-01;128(2):196-205. 2009-10-01 [PMID: 19740376] (Control, Mouse)
• Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC. Int. J. Cancer, 2009-07-01;125(1):212-21. 2009-07-01 [PMID: 19358282] (Control, Human)
• Ahern D, Lloyd CM, Robinson DS Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors. Allergy, 2009-02-07;64(8):1121-9. 2009-02-07 [PMID: 19208087] (Flow Cytometry, Human)