![Flow Cytometry: Rat IgG2a Isotype Control (KLH/G2a-1-1) [NBP1-51104] - A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/Rat-IgG2a-Isotype-Control-KLH-G2a-1-1-Flow-Cytometry-NBP1-51104-img0001.jpg "Flow Cytometry: Rat IgG2a Isotype Control (KLH/G2a-1-1) [NBP1-51104] - A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.")
| Reactivity | RtSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC, Ctrl |
| Clone | KLH/G2a-1-1 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Low Endotoxin and Azide Free |
| Concentration | Please contact technical services for concentration. |
| Description | This Rat IgG2A (KLH/G2a-1-1) immunoglobulin is useful as an isotype control for Rat IgG2A antibody. |
| Immunogen | KLH |
| Specificity | KLH |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at -20C. |
| Buffer | PBS. |
| Preservative | No Preservative |
| Concentration | Please contact technical services for concentration. |
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![Immunofluorescence: Rat IgG2a Isotype Control (KLH/G2a-1-1) [NBP1-51104] - Frozen BALB/c mouse intestine tissue was stained with Rat IgG2a-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.](http://images.novusbio.com/fullsize/Rat-IgG2a-Isotype-Control-KLH-G2a-1-1-Immunofluorescence-NBP1-51104-img0002.jpg "Immunofluorescence: Rat IgG2a Isotype Control (KLH/G2a-1-1) [NBP1-51104] - Frozen BALB/c mouse intestine tissue was stained with Rat IgG2a-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.")