Mouse IgG2a Isotype Control (C1.18.4)

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Flow Cytometry: Mouse IgG2a Isotype Control (C1.18.4) [NBP2-14864] - Cell surface flow analysis of HLA-DR in human lymphocytes using at 1 ug/10^6 cells. Cells were stained with primary antibody followed by a ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
C1.18.4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Mouse IgG2a Isotype Control (C1.18.4) Summary

Description
This Mouse IgG2A (C1.18.4) immunoglobulin is useful as an isotype control for IgG2A (C1.18.4) antibody. The C1.18.4 hybridoma cell line was grown and tissue culture supernatant was purified using Protein G.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Affinity purified
Endotoxin Note
<2.0 EU/mg as determined using an LAL gel clotting test.

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.05% BSA, PBS, pH 7
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Mouse IgG2a Isotype Control (C1.18.4)

  • IgG2A

Background

A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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