Mouse IgG2a ELISA Kit (Colorimetric)

ELISA: Mouse IgG2a ELISA Kit (Colorimetric) [NBP2-62154] - A typical standard curve for IgG2a ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Discontinued

Mouse IgG2a ELISA Kit (Colorimetric) Summary

• Specificity: Crossreactivity: Mouse IgG2a (100%), mouse IgG3 (0.49%), mouse IgM (0.12%) and <0.01%: rat IgG2a, mouse IgG1, mouse IgG2b
• Standard Curve Range: 7.81 - 250 ng/ml
• Sensitivity: 318.8 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: Low Concentration %CV - 4.6% Medium Concentration %CV - 4.8% High Concentration %CV - 4.1%
• Intra-Assay: Low Concentration %CV - 1.8% Medium Concentration %CV - 1.2% High Concentration %CV - 2.1%
• Spike Recovery: Mouse Serum - 98.4%
  Tissue Culture Media - 98.2%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Reactivity Notes

Crossreactivity: Mouse IgG2a (100%), mouse IgG3 (0.49%), mouse IgM (0.12%) and <0.01%: rat IgG2a, mouse IgG1, mouse IgG2b

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse IgG2a ELISA Kit (Colorimetric)

• IgG2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IgG2a ELISA Kit (NBP2-62154). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.