Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647]

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Flow Cytometry: Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [Alexa Fluor (R) 647] [NBP1-43319AF647] - Mouse IgG1 isotype control was used as a negative control. Image from verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IP
Clone
P3.6.2.8.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647] Summary

Description
This Mouse IgG1 Kappa immunoglobulin is useful as an isotype control.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Tested for Flow analysis on normal human peripheral blood cells and mouse spleen cells. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-43319AF647 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50 mM Sodium Borate.
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Review for Mouse IgG1, kappa L Chain Isotype Control (NBP1-43319AF647) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-43319AF647:
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Images Ratings Applications Species Date Details
Flow Cytometry IgG1 Kappa Light Chain NBP1-43319AF647
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reviewed by:
Anonymous
Flow Human 12/08/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

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Video Protocols

WB Video Protocol

FAQs for Mouse IgG1, kappa L Chain Isotype Control (NBP1-43319AF647). (Showing 1 - 1 of 1 FAQ).

  1. What is the concentration of NBP1-43319AF647?
    • All of our directly conjugated antibodies are made to order and will have the final concentration measured at the completion of this process and will be listed on the product label. This is why our concentrations are not labeled on our datasheet. For item NBP1-43319AF647, the expected concentration range for the final product should be between 0.4-0.5mg/ml; with a total volume of 1.0ml.

Other Available Formats

Alexa Fluor 700 Labeled NBP1-43319AF700

Additional IgG1 Kappa Light Chain Products

IgG1 Kappa Light Chain NBP1-43319AF647

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Anonymous
12/08/2014
Application: Flow
Species: Human