Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [DyLight 350] Summary
Description |
This Mouse IgG1 Kappa immunoglobulin is useful as an isotype control. |
Isotype |
IgG1 Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Applications/Dilutions
Dilutions |
- Control
- Flow Cytometry 1:10 - 1:1000
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- Immunoprecipitation 1:10 - 1:500
- Western Blot 1:100 - 1:2000
|
Application Notes |
Tested for Flow analysis on normal human peripheral blood cells and mouse spleen cells. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. |
Buffer |
50 mM Sodium Borate. |
Preservative |
0.05% Sodium Azide |
Notes
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for IgG1 Kappa Light Chain Isotype Control (NBP1-43319UV). (Showing 1 - 1 of 1 FAQ).
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I have a customer wanting to know the concentration for NBP1-43319UV (igg1 control [DyLight 350]) with lot# E042481633-UV.
- Prior to conjugation, this antibody is at a concentration of 0.5mg/ml. We assume no more than a 20% loss during the conjugation reaction, and as such the concentration of the conjugated product can be assumed to be at approximately 0.5mg/ml.
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