Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647]

Flow Cytometry: Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [Alexa Fluor (R) 647] [NBP1-43319AF647] - Mouse IgG1 isotype control was used as a negative control. Image from verified customer review.

• Reactivity: Mu
• Applications: WB, Flow, ICC/IF, IHC, IP, Ctrl
• Clone: P3.6.2.8.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647] Summary

• Description: This Mouse IgG1 Kappa immunoglobulin is useful as an isotype control.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Control
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Tested for Flow analysis on normal human peripheral blood cells and mouse spleen cells. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50 mM Sodium Borate.
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Review for IgG1 Kappa Isotype Control (NBP1-43319AF647) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 12/08/2014

Summary

• Application: Flow Cytometry
• Sample Tested: Human PBMC
• Species: Human

FAQs for IgG1 Kappa Isotype Control (NBP1-43319AF647). (Showing 1 - 1 of 1 FAQ).

1. What is the concentration of NBP1-43319AF647?
   • All of our directly conjugated antibodies are made to order and will have the final concentration measured at the completion of this process and will be listed on the product label. This is why our concentrations are not labeled on our datasheet. For item NBP1-43319AF647, the expected concentration range for the final product should be between 0.4-0.5mg/ml; with a total volume of 1.0ml.