Mouse IgG1 Isotype Control [Alexa Fluor® 546]

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Alexa Fluor 546

Mouse IgG1 Isotype Control [Alexa Fluor® 546] Summary

• Description: This Mouse IgG1 immunoglobulin is useful as an isotype control for Mouse IgG1 antibody.
• Isotype: IgG1
• Clonality: Polyclonal
• Host: Mouse
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) 1:10 - 1:1000
  • Flow (Intracellular) 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 5 ug/ml
• Application Notes: Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol.
• Preservative: No Preservative
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Alternate Names for Mouse IgG1 Isotype Control [Alexa Fluor® 546]

• IgG1
• Igh-4
• RGD1359539
• VH7183

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.