Mouse IgG1 Isotype Control (MOPC-21)

Flow Cytometry: Mouse IgG1 Isotype Control (MOPC-21) [NBP2-36577] - Analysis result with 3 human cancer lines. Image from verified customer review. Image using the FITC form of this antibody.

• Reactivity: Mu
• Applications: Flow
• Clone: MOPC-21
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Mouse IgG1 Isotype Control (MOPC-21) Summary

• Description: This Mouse IgG1 (MOPC-21) immunoglobulin is useful as an isotype control for Mouse IgG1 (MOPC-21) antibody.
• Specificity: This antibody has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS, approx. pH 6.5
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Mouse IgG1 Isotype Control (MOPC-21)

• IgG1
• Igh-4
• RGD1359539
• VH7183

Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ug/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG1 Isotype Control (NBP2-36577)(1)

Publication using NBP2-36577

• Geller DS, Morris J, Revskaya E et al. Targeted therapy of osteosarcoma with radiolabeled monoclonal antibody to an insulin-like growth factor-2 receptor (IGF2R) Nucl. Med. Biol. 2016-12-01 [PMID: 27744117] (FLOW, Mouse)