Non-species specific IgG1 Isotype Control (MOPC-21) [FITC]

Product Discontinued

Non-species specific IgG1 Isotype Control (MOPC-21) [FITC] Summary

• Description: The MOPC-21 is of Mouse IgG1 Kappa isotype and was chosen as an isotype control after screening on variety of resting, activated, live and fixed Rat and Human tissues.
• Specificity: The MOPC-21 immunoglobulin has unknown specificity.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
• Application Notes: Useful for negative control. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Non-species specific IgG1 Isotype Control (MOPC-21) [FITC]

• IgG1
• Igh-4
• RGD1359539
• VH7183

Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ug/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Reviews for IgG1 Isotype Control (NBP2-13183) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Other.

reviewed by: A G 01/31/2017

Summary

• Lot: 525522

reviewed by: A G Flow Other 03/01/2016

Summary

• Application: Flow Cytometry
• Sample Tested: Dog whole blood
• Species: Other
• Lot: 525522

reviewed by: Ming Lu Flow Human 05/05/2014

Summary

• Application: Flow Cytometry
• Sample Tested: 3 human cancer cell lines
• Species: Human
• Lot: 518369