Mouse IgG1 Isotype Control (MOPC-21) [FITC]

Images

 
Flow Cytometry: Mouse IgG1 Kappa Light Chain Isotype Control (MOPC-21) [FITC] [NBP2-13183] - Flow cytometry result with 3 human cancer lines. Image from verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC-Fr, IHC-P, IP, Ctrl
Clone
MOPC-21
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Mouse IgG1 Isotype Control (MOPC-21) [FITC] Summary

Description
The MOPC-21 is of Mouse IgG1 Kappa isotype and was chosen as an isotype control after screening on variety of resting, activated, live and fixed Rat and Human tissues.
Specificity
The MOPC-21 immunoglobulin has unknown specificity.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGHG1
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Useful for negative control. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 3 Reviews rated 5
using
NBP2-13183 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Mouse IgG1 Isotype Control (MOPC-21) [FITC]

  • IgG1

Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ug/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG1 Isotype Control (NBP2-13183) (0)

There are no publications for IgG1 Isotype Control (NBP2-13183).
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Reviews for IgG1 Isotype Control (NBP2-13183) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.
We have 3 reviews tested in 2 application: ICC/IF, Flow.

Reviews using NBP2-13183:
Filter by Applications
ICC/IF
(1)
Flow
(2)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence IgG1 NBP2-13183
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reviewed by:
A G
ICC/IF 01/31/2017
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Lot525522
Flow Cytometry IgG1 NBP2-13183
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reviewed by:
A G
Flow Other 03/01/2016
View

Summary

ApplicationFlow Cytometry
Sample TestedDog whole blood
SpeciesOther
Lot525522

Blocking

Blocking DetailsNA

Primary Anitbody

Dilution Ratio1ul of the stock

Details

Detection NotesUsed FACS Calibur
Fixation DetailsIncubated with Isotope for 30 mins at 4 degrees C
Permeabilization DetailsNA
Wash DescriptionWash 3 time with PBS
Flow Cytometry IgG1 NBP2-13183
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reviewed by:
Ming Lu
Flow Human 05/05/2014
View

Summary

ApplicationFlow Cytometry
Sample Tested3 human cancer cell lines
SpeciesHuman
Lot518369
CommentsThis mouse IgG1 isotype control [FITC] antibody is a very clean one. I tested 10+ human cancer lines and got good results--low and stable FL1-H values between cell lines. Picture show 3 selected lines.

Blocking

Blocking Details2% BSA in PBS buffer.

Primary Anitbody

Dilution Ratio1:200 diluted with 2%BSA-PBS, incubated on ice in dark for 30min.

Details

Detection NotesThis is used as isotype control, FL1-H value was read by flow cytometry.
Fixation DetailsNo Fixation. After deteched by versene, cells were stained alive.
Permeabilization DetailsNo Permeabilization step, cells were stained alive.
Wash Description2% BSA in PBS buffer washed 2 times.

Comments

CommentsThis mouse IgG1 isotype control [FITC] antibody is a very clean one. I tested 10+ human cancer lines and got good results--low and stable FL1-H values between cell lines. Picture show 3 selected lines.

Product General Protocols

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Video Protocols

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FAQs for IgG1 Isotype Control (NBP2-13183) (0)

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

A G
01/31/2017
Application: ICC/IF
Species:

A G
03/01/2016
Application: Flow
Species: Other

Ming Lu
05/05/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol IGHG1
Entrez