Rat IgG1 Isotype Control (HRPN) [HRP]

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Flow Cytometry: Rat IgG1 Isotype Control (HRPN) [HRP] [NBP2-30350H] - Cell surface flow analysis of 10^6 mouse splenocytes using 0.25 ug of rat isotype control. The shaded histogram represents mouse splenocytes alone; ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow
Clone
HRPN
Clonality
Monoclonal
Host
Rat
Conjugate
HRP
Concentration
0.5 mg/ml

Order Details

Rat IgG1 Isotype Control (HRPN) [HRP] Summary

Description
This Rat IgG1 (HRPN) immunoglobulin is useful as an isotype control for Rat IgG1 (HRPN) antibody. The HRPN hybridoma cell line was grown and tissue culture supernatant.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Purity
Affinity purified

Applications/Dilutions

Application Notes
Useful for Flow, IHC, and IF. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS, 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Rat IgG1 Isotype Control (HRPN) [HRP]

  • IgG1

Background

A rat isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific rat antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens. IMGENEX offers a variety of rat isotype controls including fluorescene conjugates and azide-free formats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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