Rat IgG1 Isotype Control (43414R) [Unconjugated]

• Reactivity: Rt
• Applications: Ctrl
• Clone: 43414R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Rat IgG1 Isotype Control (43414R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: KLH
• Specificity: Selected for use as rat IgG₁ isotype control. In direct ELISAs, no cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha , rrCINC-2 beta , rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rhFractalkine, rmFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha , rhGRO beta , rhGRO gamma , rhHCC-1, rhHCC-4, rhI-309, rhIL-8, rhIL-16, rhIP-10, rmJE, rmKC, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-1 R, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha , rmMIP-1 alpha , rmMIP-1 beta , rmMIP-1 gamma , rhMIP-1δ, rmMIP-2, rhMIP-3 alpha , rrMIP-3 alpha , rhMIP-3 beta , rmMIP-3 beta , recombinant viral (rv) MIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha , rmSDF-1 alpha , rhSDF-1 beta , rhTarc, rmTCA-3, rhTeck, or rmTeck.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Control

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IgG1 Isotype Control (43414R) [Unconjugated]

• IgG1
• Igh-4
• RGD1359539
• VH7183

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG1 Isotype Control (MAB005R)(2)

Publications using MAB005R

• Y Du, Q Peng, D Cheng, T Pan, W Sun, H Wang, X Ma, R He, H Zhang, Z Cui, X Feng, Z Liu, T Zhao, W Hu, L Shen, W Jiang, N Gao, BN Martin, CJ Zhang, Z Zhang, C Wang Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing gammadelta T cells Nature Communications, 2022-01-11;13(1):231. 2022-01-11 [PMID: 35017553] (In Vivo, Mouse)
• Ling Li, Xiaojun Sun, Sisi Wu, Xin Yuan, Bingxin Liu, Xueping Zhou Interleukin-12 exacerbates symptoms in an MRL/MpJ-Faslpr mouse model of systemic lupus erythematosus Experimental and Therapeutic Medicine 2021-04-15 [PMID: 33936283]