Mouse IgG1 ELISA Kit (Colorimetric)

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ELISA: Mouse IgG1 ELISA Kit (Colorimetric) - Standard curve of Mouse IgG1 ELISA Kit (Colorimetric)

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and culture supernatants of mouse origin
Standard Curve Range
7.81 - 250 ng/ml
Sensitivity
0.116 ng/mL

Order Details

Mouse IgG1 ELISA Kit (Colorimetric) Summary

Standard Curve Range
7.81 - 250 ng/ml
Sensitivity
0.116 ng/mL
Assay Type
Competitive ELISA
Inter-Assay
Mean: CV% 5.2
Intra-Assay
Mean: CV% 2.5
Spike Recovery
Mouse Serum - 102.8%
Tissue Culture Media - 105.3%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Assay Buffer 13 Concentrate
  2. Mouse IgG1 Conjugate
  3. Mouse IgG1 Microtiter Plate
  4. Mouse IgG1 Standard
  5. Stop Solution 2
  6. TMB Substrate
  7. Wash Buffer Concentrate

Alternate Names for Mouse IgG1 ELISA Kit (Colorimetric)

  • IgG1
  • Igh-4
  • RGD1359539
  • VH7183

Background

Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (Y) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. We offer isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for IgG1 ELISA Kit (NBP3-07977). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

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