Human IgG1 ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
0.05 to 40 ng/mL |
| Sensitivity |
0.05 ng/mL |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
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| Application Notes |
IgG1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IgG1. |
| Publications |
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Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent (Item E2): 5x concentrated buffer.
- Detection Antibody IgG1 (Item F): Biotinylated anti-Human IgG1. Each vialis enough to assay half the microplate.
- HRP-Streptavidin Concentrate (Item G): 2,000x concentratedHRP-conjugated streptavidin.
- IgG1 Microplate (Item A): Coated with anti-human IgG1.
- Standard Protein (Item C): Human IgG1. 1 vial is enough to run eachstandard in duplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’- tetramethylbenzidine(TMB) in buffer solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human IgG1 ELISA Kit (Colorimetric)
Background
IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IgG1 ELISA Kit (KA1730). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.