Sheep anti-Bovine IgG1 Secondary Antibody (Azide Free)

Product Discontinued

Sheep anti-Bovine IgG1 Secondary Antibody (Azide Free) Summary

• Immunogen: Pools of purified normal IgG1 isolated from pooled bovine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Clonality: Polyclonal
• Host: Sheep
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunoblotting 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Whole Antiserum
• Reconstitution Instructions: Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Alternate Names for Sheep anti-Bovine IgG1 Secondary Antibody (Azide Free)

• IgG1
• Igh-4
• RGD1359539
• VH7183

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.