Rabbit anti-Mouse IgG1 Secondary Antibody [HRP]

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Western Blot: Rabbit anti-Mouse IgG1 Secondary Antibody [HRP] [NBP1-73741] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP
Concentration
LYOPH

Rabbit anti-Mouse IgG1 Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG1 heavy chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA against other mouse or human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is negligible (<0.5%). Cross reactivity with Mouse IgG2a and IgM is minimal.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:200000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Paraffin 1:500 - 1:2500
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit anti-Mouse IgG1 Secondary Antibody [HRP]

  • IgG1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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