Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Biotin]

ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Biotin] [NBP1-73698] - ELISA Results of Rabbit anti-Mouse IgG1 Heavy Chain Heavy Chain Secondary antibody [Biotin] tested against purified Mouse IgG1 Heavy Chain Biotin. Each well was coated in duplicate with 1.0 ug of Mouse IgG1 Heavy Chain. The working dilution is 1:146,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Streptavidin-HRP conjugated and TMB substrate.

• Reactivity: Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Biotin
• Concentration: LYOPH

Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Biotin] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum
• Immunogen: Mouse IgG1 heavy chain
• Specificity: Specificity was confirmed by ELISA. Typically, less than 1% cross-reactivity was observed against other Mouse heavy chain isotypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:250000
  • Immunohistochemistry 1:500 - 1:3000
  • Immunohistochemistry-Paraffin 1:500 - 1:3000
  • Western Blot 1:1000 - 1:5000
• Application Notes: This product is available in a variety of formats. Anti-Mouse IgG1 Biotin Antibody has been tested by ELISA and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG1 Antibody (NBP1-73698)(1)

Publication using NBP1-73698

• Strait Rt, Posgai Mt, Mahler A et al. IgG1 protects against renal disease in a mouse model of cryoglobulinaemia. Nature. [PMID: 25363774] (IHC-P)
  
  Details:
  Citation using the Biotin form of this antibody.